Because of a snowstorm your regular laboratory session was cancled

Account for this result. To prepare the slides, a drop of water on each individual slide, and then each organism was transferred separately to the drop of water with a sterile, cooled loop.

The primary stain is used to stain all cells in the sample b. Last updated on 7-April at 2: The crystal violet stain was used to stain all cells purple. The ethyl alcohol was slowly added drop by drop until the alcohol ran almost clear and showed a blue tinge. The counterstain is used to stain those cells that have been previously decolorized.

Why is it essential that the primary stain and the counterstain be of contrasting colors? The three slides were finally examined under oil immersion. Examination of the stained B.

Works Cited Cappuccino, James G. The slides were labeled A S. To sterilize the loop, the coil was placed through the blame of a Bunsen burner until it turned red, and then allowed to cool.

Both organisms were mixed and spread by means of a circular motion of the cooled inoculating loop. It binds to the primary stain and forms an insoluble complex. It was important to not allow the stain to sit for too long, as it could have resulted in over-staining.

Microscope The following procedure was followed: In cultures that are older than 24 hours, the gram positive organisms may lose their ability to retain the primary stain.

Then, using aseptic technique, a smear of S.

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It was important not to over-decolorize the smears. To begin the experiment, eight clean glass slides were obtained. Because of a snowstorm, your regular laboratory session was canceled and the Gram staining procedure was performed on cultures incubated for a longer period of time.

The primary stain and counterstain need to be of contrasting color to help visualize the number cells that retained the primary stain. Which is the most critical step in the performance of the Gram staining procedures?

Cite the purpose of each of the following reagents in a differential staining procedure. The slides were rinsed again, and then counterstained with safranin for 45 seconds. The ethyl alcohol served as a protein-dehydrating agent and as a lipid solvent.

Some of the gram positive cells may appear purple and others purple. The necks of the test tubes containing the cultures were flamed as well to prevent cross-contamination.

Differential staining procedures can help identify the presence of different organisms in a culture specimen. Safranin is used to stain pink the cells that were decolorized. The slides were rinsed once more and then blotted dry with bibulous paper. What are the advantages of differential staining procedures over the simple staining technique?

It binds the primary stain and forms an insoluble complex. The resultant complex serves to intensify the color of the stain. The decolorizing agent is used to remove the color from the cells that have a thinner peptidoglycan cell wall. Some of the organisms will stain with the primary stain color and others with the counterstain color.Jun 08,  · Because of snowstorm, your regular laboratory session was canceled and the Gram staining procedure was perform on cultures incubated for a longer period of time.

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Examination of stained bacillus cereus slides revealed a great deal of color variability, ranging from an intense blue to Status: Resolved. Because of a snowstorm your regular laboratory session was cancelled and the gram staining procedure was performed on cultures incubated for a longer period of time.

Because of snowstorm, your regular laboratory session was canceled and the Gram staining procedure was perform on cultures incubated for a longer period of time. Examination of stained Bacillus Cereus slides revealed a great deal of color variability, ranging from an intense blue to shades of pink.

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Because of a snowstorm your regular laboratory session was cancelled and the gram staining was performed on cultures incubated for a longer period of time. Nov 08,  · because of the unforseen accident, your regular laboratory session was cancelled and the gram staining procedure was performed on cultures incubated for a longer period of time.

examinations of the stained bacillus cerus slides revealed a great deal of colour variability, ranging from intense blue to mint-body.com account or phenonomen? why?Status: Resolved.

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Because of a snowstorm your regular laboratory session was cancled
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